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Publication details

Document type
Journal articles

Document subtype
Full paper

Title
A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit

Participants in the publication
A. Alcântara (Author)
R. S. Morgado (Author)
S. Silvestre (Author)
J. Marques da Silva (Author)
A. Bernardes da Silva (Author)
Dep. Biologia Vegetal
 BioISI
 P. Fevereiro (Author)
S. S. Araújo (Author)

Summary
Phenotypic screening after transformation experiments aiming to identify lines with the enhanced/desired trait is still a time consuming process for most agricultural crops, especially when dealing with complex physiological responses such as water deficit. In this study we evaluated the suitability of non-destructive leaf gas-exchange analysis and imaging-PAM chlorophyll a fluorescence to select transgenic lines of Medicago truncatula expressing the Trehalose-6-Phosphate Synthase 1 (AtTPS1) from Arabidopsis thaliana with altered response to water deficit (WD) and WD recovery (WDR) in the early stages of the transformation process (T0). Primary transformants (T0) with different expression levels of a constitutive AtTPS1 construct were used. Additionally, we evaluated if the expression of the transgene could be correlated with the phenotype assessed. Among tested techniques and parameters measured, the net carbon assimilation (A) from gas-exchange analysis was the best parameter to early detect lines with WD and WDR improved performance, at the earliest stages of the transformation process. With this multidisciplinary approach, we selected 3 transgenic lines TPS7, TPS10 and TPS16 for further studies, which have higher or intermediate expression levels of the transgene and improved response to WD and WDR. This work will contribute to speed-up the identification of elite lines with confidence within a large number of individuals, thus reducing time, cost and labor associated with this plant improvement strategy.\n

Date of Submisson/Request
2015-03-17
Date of Acceptance
2015-05-10
Date of Publication
2015-05-19

Institution
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA

Where published
Plant Cell, Tissue and Organ Culture (PCTOC)

Publication Identifiers
ISSN - 0167-6857
eISSN - 01676857, 15735044

Publisher
Springer Science and Business Media LLC

Volume
122
Number
3

Number of pages
12
Starting page
605
Last page
616

Document Identifiers
DOI - https://doi.org/10.1007/s11240-015-0793-4
URL - https://doi.org/10.1007/s11240-015-0793-4


Export

APA
A. Alcântara, R. S. Morgado, S. Silvestre, J. Marques da Silva, A. Bernardes da Silva, P. Fevereiro, S. S. Araújo, (2015). A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit. Plant Cell, Tissue and Organ Culture (PCTOC), 122, 605-616. ISSN 0167-6857. eISSN 01676857, 15735044. https://doi.org/10.1007/s11240-015-0793-4

IEEE
A. Alcântara, R. S. Morgado, S. Silvestre, J. Marques da Silva, A. Bernardes da Silva, P. Fevereiro, S. S. Araújo, "A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit" in Plant Cell, Tissue and Organ Culture (PCTOC), vol. 122, pp. 605-616, 2015. 10.1007/s11240-015-0793-4

BIBTEX
@article{66130, author = {A. Alcântara and R. S. Morgado and S. Silvestre and J. Marques da Silva and A. Bernardes da Silva and P. Fevereiro and S. S. Araújo}, title = {A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit}, journal = {Plant Cell, Tissue and Organ Culture (PCTOC)}, year = 2015, pages = {605-616}, volume = 122 }