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Detalhes Referência

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit

Participantes na publicação
A. Alcântara (Author)
R. S. Morgado (Author)
S. Silvestre (Author)
J. Marques da Silva (Author)
A. Bernardes da Silva (Author)
Dep. Biologia Vegetal
 BioISI
 P. Fevereiro (Author)
S. S. Araújo (Author)

Resumo
Phenotypic screening after transformation experiments aiming to identify lines with the enhanced/desired trait is still a time consuming process for most agricultural crops, especially when dealing with complex physiological responses such as water deficit. In this study we evaluated the suitability of non-destructive leaf gas-exchange analysis and imaging-PAM chlorophyll a fluorescence to select transgenic lines of Medicago truncatula expressing the Trehalose-6-Phosphate Synthase 1 (AtTPS1) from Arabidopsis thaliana with altered response to water deficit (WD) and WD recovery (WDR) in the early stages of the transformation process (T0). Primary transformants (T0) with different expression levels of a constitutive AtTPS1 construct were used. Additionally, we evaluated if the expression of the transgene could be correlated with the phenotype assessed. Among tested techniques and parameters measured, the net carbon assimilation (A) from gas-exchange analysis was the best parameter to early detect lines with WD and WDR improved performance, at the earliest stages of the transformation process. With this multidisciplinary approach, we selected 3 transgenic lines TPS7, TPS10 and TPS16 for further studies, which have higher or intermediate expression levels of the transgene and improved response to WD and WDR. This work will contribute to speed-up the identification of elite lines with confidence within a large number of individuals, thus reducing time, cost and labor associated with this plant improvement strategy.\n

Data de Submissão/Pedido
2015-03-17
Data de Aceitação
2015-05-10
Data de Publicação
2015-05-19

Instituição
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA

Suporte
Plant Cell, Tissue and Organ Culture (PCTOC)

Identificadores da Publicação
ISSN - 0167-6857
eISSN - 01676857, 15735044

Editora
Springer Science and Business Media LLC

Volume
122
Fascículo
3

Número de Páginas
12
Página Inicial
605
Página Final
616

Identificadores do Documento
DOI - https://doi.org/10.1007/s11240-015-0793-4
URL - https://doi.org/10.1007/s11240-015-0793-4


Exportar referência

APA
A. Alcântara, R. S. Morgado, S. Silvestre, J. Marques da Silva, A. Bernardes da Silva, P. Fevereiro, S. S. Araújo, (2015). A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit. Plant Cell, Tissue and Organ Culture (PCTOC), 122, 605-616. ISSN 0167-6857. eISSN 01676857, 15735044. https://doi.org/10.1007/s11240-015-0793-4

IEEE
A. Alcântara, R. S. Morgado, S. Silvestre, J. Marques da Silva, A. Bernardes da Silva, P. Fevereiro, S. S. Araújo, "A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit" in Plant Cell, Tissue and Organ Culture (PCTOC), vol. 122, pp. 605-616, 2015. 10.1007/s11240-015-0793-4

BIBTEX
@article{66130, author = {A. Alcântara and R. S. Morgado and S. Silvestre and J. Marques da Silva and A. Bernardes da Silva and P. Fevereiro and S. S. Araújo}, title = {A method to identify early-stage transgenic Medicago truncatula with improved physiological response to water deficit}, journal = {Plant Cell, Tissue and Organ Culture (PCTOC)}, year = 2015, pages = {605-616}, volume = 122 }