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Publication details

Document type
Other

Document subtype
Preprint

Title
The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1

Participants in the publication
Bruno Carmona (Author)
Carolina Camelo (Author)
Manon Mehraz (Author)
Michel Lemullois (Author)
Mariana Lince Faria (Author)
Étienne Coyaud (Author)
H. Susana Marinho (Author)
Dep. Química e Bioquímica
 João Gonçalves (Author)
Sofia Nolasco (Author)
Francisco Pinto (Author)
Brian Raught (Author)
Anne-Marie Tassin (Author)
France Koll (Author)
Helena Soares (Author)

Summary
Centrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks.

Date of Publication
2023-07-26

Institution
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA

Where published
bioRxiv

Publication Identifiers

Publisher
Cold Spring Harbor Laboratory

Document Identifiers
DOI - https://doi.org/10.1101/2023.07.26.549647
URL - http://dx.doi.org/10.1101/2023.07.26.549647


Export

APA
Bruno Carmona, Carolina Camelo, Manon Mehraz, Michel Lemullois, Mariana Lince Faria, Étienne Coyaud, H. Susana Marinho, João Gonçalves, Sofia Nolasco, Francisco Pinto, Brian Raught, Anne-Marie Tassin, France Koll, Helena Soares, (2023). The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1

IEEE
Bruno Carmona, Carolina Camelo, Manon Mehraz, Michel Lemullois, Mariana Lince Faria, Étienne Coyaud, H. Susana Marinho, João Gonçalves, Sofia Nolasco, Francisco Pinto, Brian Raught, Anne-Marie Tassin, France Koll, Helena Soares, "The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1", 2023

BIBTEX
@misc{60649, author = {Bruno Carmona and Carolina Camelo and Manon Mehraz and Michel Lemullois and Mariana Lince Faria and Étienne Coyaud and H. Susana Marinho and João Gonçalves and Sofia Nolasco and Francisco Pinto and Brian Raught and Anne-Marie Tassin and France Koll and Helena Soares}, title = {The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1}, year = 2023 }