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Publication details

Document type
Journal articles

Document subtype
Full paper

Title
New in vitro studies on the bioprofile of genista tenera antihyperglycemic extract

Participants in the publication
Daniela Batista (Author)
Dep. Química e Bioquímica
 Pedro L. Falé (Author)
Maria L. Serralheiro (Author)
Maria E. Araújo (Author)
Paulo J. A. Madeira (Author)
Carlos Borges (Author)
Dep. Química e Bioquímica
 CQB
 Isabel Torgal (Author)
Margarida Goulart (Author)
Jorge Justino (Author)
Alice Martins (Author)
Amélia P. Rauter (Author)
Dep. Química e Bioquímica
 CQB
Summary
The inhibition of α-glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera (Fabaceae). This extract decreased α-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25 %, respectively, being more effective than acarbose, and phlorizin, the positive controls, which reduced enzymes activities only to 17.39 and 96.06 %. Once inflammation and oxidative stress are related to diabetic impairments, the anti-inflammatory activity of the extract was also evaluated, through its inhibitory activity over COX-1 enzyme (47.5 % inhibition). Moreover, after induction of oxidative stress by UV radiation, the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher (67.82 %) than that promoted by ascorbic acid, the positive control (45.05 %). In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC–DAD-ESI–MS/MS. Flavonoid diglycosides were identified as the main constituents of the extract, and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.

Date of Publication
2015-10-22

Where published
Natural Products and Bioprospecting

Publication Identifiers
ISSN - 2192-2195,2192-2209

Publisher
Springer Science and Business Media LLC

Volume
5
Number
6

Number of pages
8
Starting page
277
Last page
285

Document Identifiers
DOI - https://doi.org/10.1007/s13659-015-0077-z
URL - http://dx.doi.org/10.1007/s13659-015-0077-z

Keywords
alpha-Glucosidase inhibition In vitro digestion HPLC-DAD-ESI-MS/MS Antioxidant activity Flavonoid glycosides

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APA
Daniela Batista, Pedro L. Falé, Maria L. Serralheiro, Maria E. Araújo, Paulo J. A. Madeira, Carlos Borges, Isabel Torgal, Margarida Goulart, Jorge Justino, Alice Martins, Amélia P. Rauter, (2015). New in vitro studies on the bioprofile of genista tenera antihyperglycemic extract. Natural Products and Bioprospecting, 5, 277-285. ISSN 2192-2195,2192-2209. eISSN . http://dx.doi.org/10.1007/s13659-015-0077-z

IEEE
Daniela Batista, Pedro L. Falé, Maria L. Serralheiro, Maria E. Araújo, Paulo J. A. Madeira, Carlos Borges, Isabel Torgal, Margarida Goulart, Jorge Justino, Alice Martins, Amélia P. Rauter, "New in vitro studies on the bioprofile of genista tenera antihyperglycemic extract" in Natural Products and Bioprospecting, vol. 5, pp. 277-285, 2015. 10.1007/s13659-015-0077-z

BIBTEX
@article{44622, author = {Daniela Batista and Pedro L. Falé and Maria L. Serralheiro and Maria E. Araújo and Paulo J. A. Madeira and Carlos Borges and Isabel Torgal and Margarida Goulart and Jorge Justino and Alice Martins and Amélia P. Rauter}, title = {New in vitro studies on the bioprofile of genista tenera antihyperglycemic extract}, journal = {Natural Products and Bioprospecting}, year = 2015, pages = {277-285}, volume = 5 }