BIBLIOS

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
New in vitro studies on the bioprofile of genista tenera antihyperglycemic extract

Participantes na publicação
Daniela Batista (Author)
Dep. Química e Bioquímica
Pedro L. Falé (Author)
Maria L. Serralheiro (Author)
Maria E. Araújo (Author)
Paulo J. A. Madeira (Author)
Carlos Borges (Author)
Dep. Química e Bioquímica
CQB
Isabel Torgal (Author)
Margarida Goulart (Author)
Jorge Justino (Author)
Alice Martins (Author)
Amélia P. Rauter (Author)
Dep. Química e Bioquímica
CQB

Resumo
The inhibition of α-glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera (Fabaceae). This extract decreased α-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25 %, respectively, being more effective than acarbose, and phlorizin, the positive controls, which reduced enzymes activities only to 17.39 and 96.06 %. Once inflammation and oxidative stress are related to diabetic impairments, the anti-inflammatory activity of the extract was also evaluated, through its inhibitory activity over COX-1 enzyme (47.5 % inhibition). Moreover, after induction of oxidative stress by UV radiation, the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher (67.82 %) than that promoted by ascorbic acid, the positive control (45.05 %). In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC–DAD-ESI–MS/MS. Flavonoid diglycosides were identified as the main constituents of the extract, and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.


Suporte
Natural Products and Bioprospecting

Identificadores da Publicação
ISSN - 2192-2195,2192-2209

Editora
Springer Science and Business Media LLC



Identificadores do Documento
DOI - https://doi.org/10.1007/s13659-015-0077-z
URL - http://dx.doi.org/10.1007/s13659-015-0077-z

Keywords
alpha-Glucosidase inhibition In vitro digestion HPLC-DAD-ESI-MS/MS Antioxidant activity Flavonoid glycosides

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