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Publication details

Document type
Book chapters


Title
Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation

Participants in the publication
Joana S. Cristóvão (Author)
BioISI - Biosystems & Integrative Sciences Institute
 Bárbara J. Henriques (Author)
Dep. Química e Bioquímica
 BioISI
 Cláudio M. Gomes (Author)
Dep. Química e Bioquímica
 BioISI
Summary
Proteins exhibit a remarkable structural plasticity and may undergo conformational changes resulting in protein misfolding both in a biological context and upon perturbing physiopathological conditions. Such nonfunctional protein conformers, including misfolded states and aggregates, are often associated to protein folding diseases. Understanding the biology of protein folding diseases thus requires tools that allow the structural characterization of nonnative conformations of proteins and their interconversions. Here we present detailed procedures to monitor protein conformational changes and aggregation based on spectroscopic and biophysical methods that include circular dichroism, ATR-Fourier-transformed infrared spectroscopy, fluorescence spectroscopy and dynamic light scattering. To illustrate the application of these methods we report to our previous studies on misfolding, aggregation and amyloid fibril formation by superoxide dismutase 1 (SOD1), a protein whose toxic deposition is implicated in the neurodegenerative disease amyotrophic lateral sclerosis (ALS).

Editor(s)
Cláudio M. Gomes

Date of Publication
2019

Institution
Biosystems & Integrative Sciences Institute, Faculdade de Ciências, Universidade de Lisboa, Portugal.

Where published
Protein Misfolding Diseases - Methods and Protocols

Publication Identifiers
ISSN - 1064-3745
ISBN - 9781493988198

Address
New York, NY

Publisher
Humana Press

Collection
Methods in Molecular Biology

Edition
2
Volume
1873

Number of pages
15
Starting page
3
Last page
18

Document Identifiers
URL - https://www.ncbi.nlm.nih.gov/pubmed/30341600
URL - https://link.springer.com/protocol/10.1007/978-1-4939-8820-4_1
DOI - https://doi.org/10.1007/978-1-4939-8820-4_1

Rankings
SCIMAGO Q3 (2018) - 0.605 - Molecular Biology

Keywords
Protein aggregation Fourier-transformed infrared spectroscopy Fluorescence Dynamic light scattering Circular dichroism Amyloid ALS Protein misfolding SOD1 Thioflavin T


Export

APA
Joana S. Cristóvão, Bárbara J. Henriques, Cláudio M. Gomes, (2019). Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation. Protein Misfolding Diseases - Methods and Protocols, 3-18

IEEE
Joana S. Cristóvão, Bárbara J. Henriques, Cláudio M. Gomes, "Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation" in Protein Misfolding Diseases - Methods and Protocols, 2019, pp. 3-18

BIBTEX
@incollection{40965, author = {Joana S. Cristóvão and Bárbara J. Henriques and Cláudio M. Gomes}, title = {Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation}, booktitle = {Protein Misfolding Diseases - Methods and Protocols}, year = 2019, pages = {3-18}, address = {New York, NY}, publisher = {Humana Press} }