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Detalhes Referência

Tipo
Capítulo em Livro


Título
Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation

Participantes na publicação
Joana S. Cristóvão (Author)
BioISI - Biosystems & Integrative Sciences Institute
 Bárbara J. Henriques (Author)
Dep. Química e Bioquímica
 BioISI
 Cláudio M. Gomes (Author)
Dep. Química e Bioquímica
 BioISI
Resumo
Proteins exhibit a remarkable structural plasticity and may undergo conformational changes resulting in protein misfolding both in a biological context and upon perturbing physiopathological conditions. Such nonfunctional protein conformers, including misfolded states and aggregates, are often associated to protein folding diseases. Understanding the biology of protein folding diseases thus requires tools that allow the structural characterization of nonnative conformations of proteins and their interconversions. Here we present detailed procedures to monitor protein conformational changes and aggregation based on spectroscopic and biophysical methods that include circular dichroism, ATR-Fourier-transformed infrared spectroscopy, fluorescence spectroscopy and dynamic light scattering. To illustrate the application of these methods we report to our previous studies on misfolding, aggregation and amyloid fibril formation by superoxide dismutase 1 (SOD1), a protein whose toxic deposition is implicated in the neurodegenerative disease amyotrophic lateral sclerosis (ALS).

Editor
Cláudio M. Gomes

Data de Publicação
2019

Instituição
Biosystems & Integrative Sciences Institute, Faculdade de Ciências, Universidade de Lisboa, Portugal.

Suporte
Protein Misfolding Diseases - Methods and Protocols

Identificadores da Publicação
ISSN - 1064-3745
ISBN - 9781493988198

Local
New York, NY

Editora
Humana Press

Coleção
Methods in Molecular Biology

Edição
2
Volume
1873

Número de Páginas
15
Página Inicial
3
Página Final
18

Identificadores do Documento
URL - https://www.ncbi.nlm.nih.gov/pubmed/30341600
URL - https://link.springer.com/protocol/10.1007/978-1-4939-8820-4_1
DOI - https://doi.org/10.1007/978-1-4939-8820-4_1

Identificadores de Qualidade
SCIMAGO Q3 (2018) - 0.605 - Molecular Biology

Keywords
Protein aggregation Fourier-transformed infrared spectroscopy Fluorescence Dynamic light scattering Circular dichroism Amyloid ALS Protein misfolding SOD1 Thioflavin T


Exportar referência

APA
Joana S. Cristóvão, Bárbara J. Henriques, Cláudio M. Gomes, (2019). Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation. Protein Misfolding Diseases - Methods and Protocols, 3-18

IEEE
Joana S. Cristóvão, Bárbara J. Henriques, Cláudio M. Gomes, "Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation" in Protein Misfolding Diseases - Methods and Protocols, 2019, pp. 3-18

BIBTEX
@incollection{40965, author = {Joana S. Cristóvão and Bárbara J. Henriques and Cláudio M. Gomes}, title = {Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation}, booktitle = {Protein Misfolding Diseases - Methods and Protocols}, year = 2019, pages = {3-18}, address = {New York, NY}, publisher = {Humana Press} }