BIBLIOS

Document type
Book chapters


Title
Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

Participants in the publication
Margarida D. Amaral (Author)
Dep. Química e Bioquímica
BioISI
Carlos M. Farinha (Author)
Dep. Química e Bioquímica
BioISI
Paulo Matos (Author)
Hugo M. Botelho (Author)
Dep. Química e Bioquímica
BioISI

Summary
Secretory traffic became a topical field because many important cell regulators are plasma membrane proteins (transporters, channels, receptors), being thus key targets in biomedicine and drug discovery. Cystic fibrosis (CF), caused by defects in a single gene encoding the CF transmembrane conductance regulator (CFTR), constitutes the most common of rare diseases and certainly a paradigmatic one.Here we focus on five different approaches that allow biochemical and cellular characterization of CFTR from its co-translational insertion into the ER membrane to its delivery to the plasma membrane.


Where published
Unconventional Protein Secretion,Methods in Molecular Biology

Publication Identifiers
ISSN - 1064-3745
ISBN - 9781493938025,9781493938049

Publisher
Springer New York



Document Identifiers
URL - http://dx.doi.org/10.1007/978-1-4939-3804-9_7
DOI - https://doi.org/10.1007/978-1-4939-3804-9_7

Rankings
SCIMAGO Q3 (2016) - 0.585 - Genetics

Keywords
Secretory traffic Biochemistry CFTR Cystic fibrosis Endoplasmic reticulum Golgi High-throughput microscopy Plasma membrane N-glycosylation ABC transporters