BIBLIOS

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
What If Root Nodules Are a Guesthouse for a Microbiome? The Case Study of Acacia longifolia

Participantes na publicação
Joana G. Jesus (Author)
CE3C
Cristina Máguas (Author)
CE3C
Ricardo Dias (Author)
CE3C
Mónica Nunes (Author)
Pedro Pascoal (Author)
Marcelo Pereira (Author)
Helena Trindade (Author)
Dep. Biologia Vegetal
CE3C

Resumo
Acacia longifolia is one of the most aggressive invaders worldwide whose invasion is potentiated after a fire, a common perturbation in Mediterranean climates. As a legume, this species establishes symbioses with nitrogen-fixing bacteria inside root nodules; however, the overall microbial diversity is still unclear. In this study, we addressed root nodules’ structure and biodiversity through histology and Next-Generation Sequencing, targeting 16S and 25S-28S rDNA genes for bacteria and fungi, respectively. We wanted to evaluate the effect of fire in root nodules from 1-year-old saplings, by comparing unburnt and burnt sites. We found that although having the same general structure, after a fire event, nodules had a higher number of infected cells and greater starch accumulation. Starch accumulated in uninfected cells can be a possible carbon source for the microbiota. Regarding diversity, Bradyrhizobium was dominant in both sites (ca. 77%), suggesting it is the preferential partner, followed by Tardiphaga (ca. 9%), a non-rhizobial Alphaproteobacteria, and Synechococcus, a cyanobacteria (ca. 5%). However, at the burnt site, additional N-fixing bacteria were included in the top 10 genera, highlighting the importance of this process. Major differences were found in the mycobiome, which was diverse in both sites and included genera mostly described as plant endophytes. Coniochaeta was dominant in nodules from the burnt site (69%), suggesting its role as a facilitator of symbiotic associations. We highlight the presence of a large bacterial and fungal community in nodules, suggesting nodulation is not restricted to nitrogen fixation. Thus, this microbiome can be involved in facilitating A. longifolia invasive success.


Suporte
Biology

Identificadores da Publicação
ISSN - 2079-7737

Editora
MDPI AG



Identificadores do Documento
DOI - https://doi.org/10.3390/biology12091168
URL - https://doi.org/10.3390/biology12091168

Identificadores de Qualidade
SCIMAGO Q1 (2022) - 0.779 - Agricultural and Biological Sciences (miscellaneous)
SCOPUS Q1 (2022) - 4 - General Agricultural and Biological Sciences