BIBLIOS

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
Combination of gold nanoparticles with near-infrared light as an alternative approach for melanoma management

Participantes na publicação
Joana Lopes (Author)
Carla M. Rodrigues (Author)
Ana Godinho-Santos (Author)
João M.P. Coelho (Author)
Dep. Física
IBEB
Luís C. Cabaço (Author)
Duarte C. Barral (Author)
Pedro Faísca (Author)
José Catarino (Author)
Daniela Nunes (Author)
Elvira Fortunato (Author)
Rodrigo Martins (Author)
Cecília M.P. Rodrigues (Author)
Maria Manuela Gaspar (Author)
Catarina Pinto Reis (Author)

Resumo
Melanoma is the most aggressive type of skin cancer and recently approved drugs are often associated with resistance and significant adverse effects. Therefore, the design of more effective and safe options remains imperative. Photothermal therapy (PTT) using gold nanoparticles (AuNPs) presents a promising and innovative approach. In this work, the efficacy of combining a previously optimized formulation of AuNPs coated with a mixture of hyaluronic and oleic acids (HAOA-AuNPs) with near-infrared (NIR) laser irradiation in melanoma cell lines was explored. Coated and uncoated AuNPs formulations were characterized in physicochemical, morphological and elemental terms. Next, the cellular uptake efficiency as well as antiproliferative activity of the combination of each formulation with laser irradiation was evaluated. Subsequently, HAOA-AuNPs were selected to assess the underlying mechanism of combined therapy by cell cycle and Annexin V/PI assays. An in vivo syngeneic murine melanoma model was also conducted. In vitro studies demonstrated that 24 h after incubation and in the absence of laser, HAOA-AuNPs did not exhibit cytotoxic effects on the melanoma cell lines tested, similar to the laser alone. On the contrary, the combination therapy resulted in a large reduction in cell viability. Furthermore, it has been shown to promote S-phase cell cycle arrest and increase in the percentage of late apoptotic cells. Finally, the in vivo proof-of-concept showed that the intratumoral administration of HAOA-AuNPs followed by three laser irradiations impaired tumor progression. Collectively, AuNP-based PTT holds significant potential to improve treatment efficacy and safety, offering a versatile and potent tool against cancer.


Suporte
International Journal of Pharmaceutics

Identificadores da Publicação
ISSN - 0378-5173

Editora
Elsevier BV


Identificadores do Documento
DOI - https://doi.org/10.1016/j.ijpharm.2024.124952
URL - http://dx.doi.org/10.1016/j.ijpharm.2024.124952

Identificadores de Qualidade
SCIMAGO Q1 (2023) - 0.95 - Phamaceutical Science
Web Of Science Q1 (2021) - 6.51 - PHARMACOLOGY & PHARMACY - SCIE