BIBLIOS

Document type
Conference papers

Document subtype
Abstract

Title
Potential of concomitant radiation therapy with gold nanoparticles for pancreatic cancer

Participants in the publication
A Martins (Author)
B. C. Ferreira (Author)
Dep. Física
IBEB
M. Manuela Gaspar (Author)
S Vieira (Author)
J Lopes (Author)
A S Viana (Author)
A Paulo (Author)
F Mendes (Author)
P Campelo (Author)
R Martins (Author)
Dep. Estatística e Investigação Operacional
CEAUL
C Reis (Author)
IBEB

Summary
Purpose/Objective:\\\\nIntroduction: Pancreatic cancer is one of the most difficult oncologic diseases with no significant improvements in\\\\ntreatment outcome for the past 40 years. Presently, 5-year survival rates are about 9%. The concomitant delivery of\\\\nGold Nanoparticles (AuNPs) during Radiation Therapy (RT) may represent an opportunity to improve the response to\\\\ntreatment for this pathology. The present work consisted of an exploratory study aiming to evaluate the in vitro\\\\npotential of AuNPs during RT towards human pancreatic adenocarcinoma cells.\\\\nMaterial/Methods:\\\\nMaterials and Methods: In this study, functionalized AuNPs with hyaluronic, and oleic acids (HAOA-AuNPs), and AuNPs\\\\nwith bombesin (BBN-AuNPs) were used. Atomic Force Microscopy (AFM) was used to characterize AuNPs in terms of\\\\ntheir mean size and morphology. Pancreatic tumour cells, BxPC-3, were seeded in 96-well plates with a concentration\\\\nof 4×104 cells/mL and allowed to adhere for 24h. Complete medium was then discarded, and cells were incubated\\\\nwith AuNPs for 4 h to allow internalization. Non-internalized AuNPs were then discarded, and 100 μL of complete\\\\nmedium was added to each well. Tumour cells were irradiated, in the absence or presence of AuNPs at concentrations\\\\nranging from 50 to 400 μM in a linear accelerator at a 10 cm depth. A 6 MV X-rays beam with 20x20 cm2 field size at\\\\na dose rate of 600 MU/min was used with doses ranging from 2 to 5 Gy. Loss in cell viability induced by AuNP alone,\\\\nRT alone, and the combined treatment was evaluated 24, 48 and 72 h after irradiation by MTT assay. Statistical analysis\\\\non cell viability was evaluated by two-way ANOVA followed by Tukey’s multiple comparisons test.\\\\nResults:\\\\nResults: AFM showed that HAOA-AuNPs and BBN-AuNPs are spherical with a mean size of 119±28 nm and 47±8 nm,\\\\nrespectively.\\\\nWith RT alone, only for the 72 h post-irradiation time, a statistically significant reduction in cell viability from 17-32%\\\\nwas obtained for cells irradiated with doses ranging from 2 to 5 Gy compared to the control (cells not irradiated), (p\\\\n≤ 0.0001). Both the treatment with HAOA-AuNPs or BBN-AuNPs alone induced cytotoxicity in a dose-dependent\\\\nmanner. The results of MTT assay performed 72 h post-incubation with HAOA-AuNPs alone tested at 200 and 400 μM\\\\nshowed a reduction statistically significant in cell viability of approximately 20±4% and 35±4%, respectively, compared\\\\nto the control (p < 0.0001). No significant difference was obtained between the treatment using HAOA-AuNPs at 50\\\\nμM and the control (p = 0.26). A reduction in cell viability of 25±3% and 37±3% compared to control was achieved 72\\\\nh after incubation with BBN-AuNPs at 50 and 200 μM, respectively (p < 0.0001).\\\\nS5371 Radiobiology - Tumour biology ESTRO 2024\\\\nAt 72 h post-irradiation, cell viability for RT+HAOA-AuNPs at 50 μM was not significantly different from RT alone (p >\\\\n0.6). By contrary, in average a statistically significant loss in cell viability between RT+HAOA-AuNPs at concentrations\\\\nof 200 μM and 400 μM and RT alone was obtained (p < 0.004), resulting in a loss in cell viability of 37%-51% compared\\\\nto control.\\\\nAlso, for the 72 h post-irradiation time, RT+BBN-AuNPs at 50 μM and 200 μM induced a mean loss in cell viability of\\\\nabout 44% when compared to control (p < 0.0001) and significantly different than RT alone (p <0.0001). However, no\\\\nstatistically significant difference was obtained between RT+BBN-AuNPs at 200 μM and AuNPs alone. For this higher\\\\nBBN-AuNP concentration, cell viability for the combined treatment seemed to be only caused by BBN-AuNPs.\\\\nConclusion:\\\\nConclusion: Post-irradiation incubation time is extremely relevant for the evaluation of cell viability by MTT assay. For\\\\nthe range of post-incubation times evaluated, the 72 h post-irradiation time proved to be most appropriate to\\\\nevaluate the effects of RT. The combination of RT+HAOA-AuNPs at high concentration led to a similar cell viability\\\\ncompared to the RT+BBN-AuNPs at a low concentration demonstrating that the particle size and/or coating of tested\\\\nnanoparticles influenced cell viability. Furthermore, the combination of RT with AuNPs led to cell viability loss\\\\nsignificantly different from the control, or RT alone, thus representing a potential anticancer benefit. Additional in\\\\nvitro studies, such as clonogenic assays, should be performed to support these findings.

Editor(s)
Elsevier


Institution
UNIVERSIDADE DE LISBOA

Event
Radiotherapy & Oncology

Publication Identifiers



Document Identifiers
URL - https://www.thegreenjournal.com/action/showPdf?pii=S0167-8140%2824%2901578-0

Rankings
SCIMAGO Q1 (2023) - 1.7 - Radiation Therapy