BIBLIOS

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
Postweaning Development Influences Endogenous VPAC1 Modulation of LTP Induced by Theta-Burst Stimulation: A Link to Maturation of the Hippocampal GABAergic System

Participantes na publicação
Marta Gil (Author)
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
BioISI - Biosystems & Integrative Sciences Institute
Ana Caulino-Rocha (Author)
BioISI - Biosystems & Integrative Sciences Institute
Unidade de I&D e Inovação
CQB
Marta Bento (Author)
BioISI - Biosystems & Integrative Sciences Institute
Nádia C. Rodrigues (Author)
INSTITUTO DE MEDICINA MOLECULAR DA UL
Armando Silva-Cruz (Author)
INSTITUTO DE MEDICINA MOLECULAR DA UL
Joaquim A. Ribeiro (Author)
INSTITUTO DE MEDICINA MOLECULAR DA UL
FACULDADE DE MEDICINA DA UL
Diana Cunha-Reis (Author)
Dep. Biologia Vegetal
BioISI

Resumo
Long-term potentiation (LTP) induced by theta-burst stimulation (TBS) undergoes postweaning developmental changes partially linked to GABAergic circuit maturation. Endogenous vasoactive intestinal peptide (VIP) acting on its VPAC1 receptor strongly influences LTP induced by theta-burst stimulation (TBS), an effect dependent on GABAergic transmission. Although VPAC1 receptor levels are developmentally regulated during embryogenesis, their variation along postweaning development is unknown, as is the VPAC1 modulation of LTP or its relation to hippocampal GABAergic circuit maturation. As such, we investigated how VPAC1 modulation of LTP adjusts from weaning to adulthood along with GABAergic circuit maturation. As described, LTP induced by mild TBS (5 bursts, 4 pulses delivered at 100 Hz) was increasingly greater from weaning to adulthood. The influence of the VPAC1 receptor antagonist PG 97-269 (100 nM) on TBS-induced LTP was much larger in juvenile (3-week-old) than in young adult (6–7-week-old) or adult (12-week-old) rats. This effect was not associated with a developmental decrease in synaptic VPAC1 receptor levels. However, an increase in pre and post-synaptic GABAergic synaptic markers suggests an increase in the number of GABAergic synaptic contacts that is more prominent than the one observed in glutamatergic connections during this period. Conversely, endogenous VPAC2 receptor activation did not significantly influence TBS-induced LTP. VPAC2 receptor levels enhance pronouncedly during postweaning development, but not at synaptic sites. Given the involvement of VIP interneurons in several aspects of hippocampal-dependent learning, neurodevelopmental disorders, and epilepsy, this could provide important insights into the role of VIP modulation of hippocampal synaptic plasticity during normal and altered brain development potentially contributing to epileptogenesis.


Instituição
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA

Suporte
Biomolecules

Identificadores da Publicação
ISSN - 2218-273X

Local
Basel, Switzerland

Editora
MDPI AG



Identificadores do Documento
DOI - https://doi.org/10.3390/biom14030379
URL - http://dx.doi.org/10.3390/biom14030379

Identificadores de Qualidade
SCOPUS Q1 (2022) - 5.5 - Biochemistry & Molecular Biology
SCIMAGO Q1 (2022) - 1.07 - Biochemistry

Keywords
VIP theta-burst LTP VPAC1 receptor hippocampus VGAT VGlut-1 gephyrin PSD-95