BIBLIOS

Document type
Journal articles

Document subtype
Full paper

Title
Environmentally Friendly Degradation and Detoxification of Rifampicin by a Bacterial Laccase and Hydrogen Peroxide

Participants in the publication
Paulo Durao (Author)
Peter Kis (Author)
Ivo M. Chelo (Author)
cE3c/FCUL
Dep. Biologia Animal
M. Rita Ventura (Author)
Lígia O. Martins (Author)

Summary
Antibiotics are micropollutants accumulating in our rivers and wastewaters, potentially leading to bacterial antibiotic resistance, a worldwide problem to which there is no current solution. Here, we have developed an environmentally friendly two-step process to transform the antibiotic rifampicin (RIF) into non-antimicrobial compounds. The process involves an enzymatic oxidation step by the bacterial CotA-laccase and a hydrogen peroxide bleaching step. NMR identified rifampicin quinone as the main product of the enzymatic oxidation. Growth of Escherichia coli strains in the presence of final degradation products (FP) and minimum inhibitory concentration (MIC) measurements confirmed that FP are non-anti-microbial compounds, and bioassays suggest that FP is not toxic to eukaryotic organisms. Moreover, competitive fitness assays between susceptible and RIF-resistant bacteria show that susceptible bacteria is strongly favoured in the presence of FP. Our results show that we have developed a robust and environmentally friendly process to effectively remediate rifampicin from antibiotic contaminated environments.


Institution
INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA

Where published
ChemBioChem

Publication Identifiers
ISSN - 1439-4227
eISSN - 1439-7633

Address
Weinheim, Alemanha

Publisher
Wiley



Document Identifiers
DOI - https://doi.org/10.1002/cbic.202300627
URL - http://dx.doi.org/10.1002/cbic.202300627

Rankings
SCIMAGO Q1 (2021) - 918 - Organic Chemistry
SCIMAGO Q1 (2019) - 1.009 - Molecular Biology (Q2)

Keywords
Antibiotics Bioremediation Chemo-enzymatic transformation CotA-laccase Hydrogen peroxide Animal toxicity

Download