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Detalhes Referência

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
Activity of ammonia-oxidizing bacteria in enriched cultures exposed to 3,4-dimethyl-1H-pyrazole dihydrogen phosphate nitrification inhibitor

Participantes na publicação
Janaina M. Rodrigues (Author)
Cristina Cruz (Author)
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
 Dep. Biologia Vegetal
 CE3C
 Rogerio Tenreiro (Author)
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
 Catarina A. Gouveia (Author)
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
 Berta Lasa (Author)

Resumo
The use of nitrification inhibitors is an interesting tool to achieve a higher N efficiency in plants while decreasing the environmental impact of N fertilization. However, an integrated evaluation of the efficiency of nitrification inhibitors over time, understood as the period in which the nitrifying activity is inhibited or slows down, is necessary to assess whether their use is ecofriendly and sustainable.\n\nTo test the direct efficiency of 3,4-dimethyl-1H-pyrazole dihydrogen phosphate (DMPP) on nitrification, a study has been carried out in two cultures enriched with ammonia-oxidizing bacteria (AOB) obtained from a soil with continuous N fertilization (80 kg N ha−1 year−1 as NH4NO3) and from soil without N fertilization. In addition, Cu has been evaluated as a cofactor of ammonia monoxygenase, a key enzyme in the nitrifying activity of AOBs. On the other hand, the stability of DMP has been studied both in the cultivation system enriched in AOBs and in soil to assess the efficiency of the inhibitor due to its persistence over time.\n\nOur work reveals that nitrification rates observed in cultures enriched in AOBs from genus Nitrosospira isolated from soils with continuous N fertilization were not higher than those of cultures without N fertilization. In AOB cultures, DMPP was a very efficient inhibitor of nitrification (> 50 % inhibition of integrated AMO activity), mainly due to the stability of DMP (3,4-dimethyl-1 H-pyrazole) in the cultures. However, DMP stability was significantly lower under soil conditions (> 90 % of DMP was degraded in the first 30 days of incubation). Other metals are suggested as cofactors of the enzyme ammonia monooxygenase alternatively to Cu.

Data de Publicação
2023-10

Instituição
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA

Suporte
European Journal of Agronomy

Identificadores da Publicação
ISSN - 1161-0301

Editora
Elsevier BV

Volume
150

Página Inicial
126946

Identificadores do Documento
DOI - https://doi.org/10.1016/j.eja.2023.126946
URL - http://dx.doi.org/10.1016/j.eja.2023.126946


Exportar referência

APA
Janaina M. Rodrigues, Cristina Cruz, Rogerio Tenreiro, Catarina A. Gouveia, Berta Lasa, (2023). Activity of ammonia-oxidizing bacteria in enriched cultures exposed to 3,4-dimethyl-1H-pyrazole dihydrogen phosphate nitrification inhibitor. European Journal of Agronomy, 150, ISSN 1161-0301. eISSN . http://dx.doi.org/10.1016/j.eja.2023.126946

IEEE
Janaina M. Rodrigues, Cristina Cruz, Rogerio Tenreiro, Catarina A. Gouveia, Berta Lasa, "Activity of ammonia-oxidizing bacteria in enriched cultures exposed to 3,4-dimethyl-1H-pyrazole dihydrogen phosphate nitrification inhibitor" in European Journal of Agronomy, vol. 150, 2023. 10.1016/j.eja.2023.126946

BIBTEX
@article{59520, author = {Janaina M. Rodrigues and Cristina Cruz and Rogerio Tenreiro and Catarina A. Gouveia and Berta Lasa}, title = {Activity of ammonia-oxidizing bacteria in enriched cultures exposed to 3,4-dimethyl-1H-pyrazole dihydrogen phosphate nitrification inhibitor}, journal = {European Journal of Agronomy}, year = 2023, volume = 150 }