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Detalhes Referência

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system

Participantes na publicação
Sang Hyun Lim (Author)
Jamie Snider (Author)
Liron Birimberg-Schwartz (Author)
Wan Ip (Author)
Joana C Serralha (Author)
BioISI - Biosystems & Integrative Sciences Institute
 Hugo M Botelho (Author)
Dep. Química e Bioquímica
 BioISI
 Miquéias Lopes-Pacheco (Author)
Dep. Química e Bioquímica
 BioISI
 Madalena C Pinto (Author)
BioISI
 Mohamed Taha Moutaoufik (Author)
Mara Zilocchi (Author)
Onofrio Laselva (Author)
Mohsen Esmaeili (Author)
Max Kotlyar (Author)
Anna Lyakisheva (Author)
Priscilla Tang (Author)
Lucía López Vázquez (Author)
Indira Akula (Author)
Farzaneh Aboualizadeh (Author)
Victoria Wong (Author)
Ingrid Grozavu (Author)
Teuta Opacak-Bernardi (Author)
Zhong Yao (Author)
Meg Mendoza (Author)
Mohan Babu (Author)
Igor Jurisica (Author)
Tanja Gonska (Author)
Christine E Bear (Author)
Margarida D Amaral (Author)
Dep. Química e Bioquímica
 BioISI
 Igor Stagljar (Author)

Resumo
Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a chloride and bicarbonate channel in secretory epithelia with a critical role in maintaining fluid homeostasis. Mutations in CFTR are associated with Cystic Fibrosis (CF), the most common lethal autosomal recessive disorder in Caucasians. While remarkable treatment advances have been made recently in the form of modulator drugs directly rescuing CFTR dysfunction, there is still considerable scope for improvement of therapeutic effectiveness. Here, we report the application of a high-throughput screening variant of the Mammalian Membrane Two-Hybrid (MaMTH-HTS) to map the protein-protein interactions of wild-type (wt) and mutant CFTR (F508del), in an effort to better understand CF cellular effects and identify new drug targets for patient-specific treatments. Combined with functional validation in multiple disease models, we have uncovered candidate proteins with potential roles in CFTR function/CF pathophysiology, including Fibrinogen Like 2 (FGL2), which we demonstrate in patient-derived intestinal organoids has a significant effect on CFTR functional expression.

Data de Publicação
2022-02

Suporte
Molecular Systems Biology

Identificadores da Publicação
ISSN - 1744-4292

Editora
EMBO

Volume
18
Fascículo
2

Identificadores do Documento
DOI - https://doi.org/10.15252/msb.202110629
URL - http://dx.doi.org/10.15252/msb.202110629

Identificadores de Qualidade
Web Of Science Q1 (2020) - 11.429 - BIOCHEMISTRY & MOLECULAR BIOLOGY - SCIE
SCIMAGO Q1 (2020) - 8.523 - Biochemistry, Genetics and Molecular Biology (miscellaneous)
SCIMAGO Q1 (2020) - 8.523 - Computational Theory and Mathematics
SCIMAGO Q1 (2020) - 8.523 - Information Systems
SCIMAGO Q1 (2020) - 8.523 - Medicine (miscellaneous)
SCOPUS Q1 (2020) - 15.6 - Applied Mathematics
SCOPUS Q1 (2020) - 15.6 - Biochemistry, Genetics and Molecular Biology (all)
SCIMAGO Q1 (2019) - 7.338 - Applied Mathematics
SCIMAGO Q1 (2019) - 7.338 - Biochemistry, Genetics and Molecular Biology (miscellaneous)
SCIMAGO Q1 (2019) - 7.338 - Computational Theory and Mathematics

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APA
Sang Hyun Lim, Jamie Snider, Liron Birimberg-Schwartz, Wan Ip, Joana C Serralha, Hugo M Botelho, Miquéias Lopes-Pacheco, Madalena C Pinto, Mohamed Taha Moutaoufik, Mara Zilocchi, Onofrio Laselva, Mohsen Esmaeili, Max Kotlyar, Anna Lyakisheva, Priscilla Tang, Lucía López Vázquez, Indira Akula, Farzaneh Aboualizadeh, Victoria Wong, Ingrid Grozavu, Teuta Opacak-Bernardi, Zhong Yao, Meg Mendoza, Mohan Babu, Igor Jurisica, Tanja Gonska, Christine E Bear, Margarida D Amaral, Igor Stagljar, (2022). CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system. Molecular Systems Biology, 18, ISSN 1744-4292. eISSN . http://dx.doi.org/10.15252/msb.202110629

IEEE
Sang Hyun Lim, Jamie Snider, Liron Birimberg-Schwartz, Wan Ip, Joana C Serralha, Hugo M Botelho, Miquéias Lopes-Pacheco, Madalena C Pinto, Mohamed Taha Moutaoufik, Mara Zilocchi, Onofrio Laselva, Mohsen Esmaeili, Max Kotlyar, Anna Lyakisheva, Priscilla Tang, Lucía López Vázquez, Indira Akula, Farzaneh Aboualizadeh, Victoria Wong, Ingrid Grozavu, Teuta Opacak-Bernardi, Zhong Yao, Meg Mendoza, Mohan Babu, Igor Jurisica, Tanja Gonska, Christine E Bear, Margarida D Amaral, Igor Stagljar, "CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system" in Molecular Systems Biology, vol. 18, 2022. 10.15252/msb.202110629

BIBTEX
@article{54537, author = {Sang Hyun Lim and Jamie Snider and Liron Birimberg-Schwartz and Wan Ip and Joana C Serralha and Hugo M Botelho and Miquéias Lopes-Pacheco and Madalena C Pinto and Mohamed Taha Moutaoufik and Mara Zilocchi and Onofrio Laselva and Mohsen Esmaeili and Max Kotlyar and Anna Lyakisheva and Priscilla Tang and Lucía López Vázquez and Indira Akula and Farzaneh Aboualizadeh and Victoria Wong and Ingrid Grozavu and Teuta Opacak-Bernardi and Zhong Yao and Meg Mendoza and Mohan Babu and Igor Jurisica and Tanja Gonska and Christine E Bear and Margarida D Amaral and Igor Stagljar}, title = {CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system}, journal = {Molecular Systems Biology}, year = 2022, volume = 18 }