Document type
Conference papers
Document subtype
Poster without full or short paper
Title
IS PENICILL/UM CRUSTOSUM A POTENCIAL OCHRATOXIN-A PRODUCER?
Participants in the publication
T. Dias (Author)
Celia Soares (Author)
Carla Santos (Author)
Margarida Barata (Author)
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
Dep. Biologia Vegetal
cE3c
N e l s o n L i m a (Author)
Summary
Mycotoxins are fungal secondary metabolites that can cause severe health issues in either humans or domesticated animals when ingested, inhaled and/or absorbed. A short list of mycotoxins with higher health risks are the most studied and Ochratoxin A (OTA) is among those. This toxin is present in a wide diversity of food and feed products [1] being Aspergillus and Penicillium species associated with the food spoilage and with OTA contamination. The major OTA producers are P. verrucosum, P. nordicum and A. carbonarius, but many other species of Aspergillus genus have that ability. Recent studies have reported the presence of OTA in food matrices where known OTA producers are not present\\n[2]. For that reason, and based on previous evidences [3], other species, such as P. crustosum, are now being considered. Therefore, the main goal of this work was to search for potential OTA producers among P. crustosum strains with different geographic origins and to search for potential genetic differences at the sub-species level.\\nA total of 44 strains of P. crustosum from different parts of the globe supplied by Micoteca da Universidade do Minho (MUM) and Colecci6n Chilena de Cultivos Tipo (CCCT) culture collections were studied. Mycotoxin production was analyzed, after 7 days growth of the fungi, by HPLC-FL. In addition, genes associated with OTA production were tested. RAPD-PCR fingerprinting (M13 and GACA4) and beta-tubulin gene sequencing were used to do a wide phylogenetic study.\\nSome genetic differences between isolates were found allowing the clustering of strains from the same geographic region, except for isolates from Italy. Under the studied conditions, preliminary results showed that there are no OTA producers above the HPLC-FL detection limit. However, further studies with a broader array of conditions need to be considered.\\n
Date of Submisson/Request
2019
Date of Acceptance
2019-06-12
Date of Publication
2019
Institution
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
Event
ECCO 2019 12th - 14th June, 2019 Torino, Italy
Publication Identifiers
Address
Torino, Italy
Organizers
University of Torino Department of Life Sciences and Systems Biology Mycotheca Universitatis Taurinensis (MUT) Turin University Culture Collection (TUCC)
Publisher
XXXVIII Annual Meeting of the European Culture Collections' Organisation
Document Identifiers