BIBLIOS

Document type
Journal articles

Document subtype
Full paper

Title
The systems V(IV)O(2+) - glutathione and related ligands: a potentiometric and spectroscopic study

Participants in the publication
João Pessoa (Author)
Isabel Tomaz (Author)
Dep. Química e Bioquímica
CQE
Tamás Kiss (Author)
Erzsébet Kiss (Author)
Péter Buglyó (Author)

Summary
The equilibria in the system V(IV)O(2+)-glutathione in aqueous solution were studied in the pH range 2–11 by a combination of pH-potentiometry and spectroscopy (EPR, visible absorption and circular dichroism). The results of the various methods are consistent and the equilibrium model includes the species MLH3, MLH2, MLH, ML2H2, MLH–1, and MLH–2 and several hydrolysis products (where H4L denotes totally protonated glutathione); individual formation constants and spectra are given. ML2H2 is the predominant species at physiological pH. Plausible structures for each stoichiometry are discussed. The related V(IV)O(2+) systems of S-methylglutathione and γ-L-glutamyl-L-cysteinyl ethyl ester were studied by means of the same spectroscopic techniques in order to support the established binding modes for the glutathione complexes. The importance of glutathione and oxidized glutathione in binding V(IV)O(2+) in cells is assessed.


Where published
JBIC Journal of Biological Inorganic Chemistry

Publication Identifiers
ISSN - 0949-8257

Publisher
Springer Science and Business Media LLC



Document Identifiers
DOI - https://doi.org/10.1007/s007750100289
URL - http://dx.doi.org/10.1007/s007750100289

Rankings
Web Of Science Q1 (2019) - 3.246 - CHEMISTRY, INORGANIC & NUCLEAR - SCIE
SCOPUS Q1 (2019) - 5.6 - Inorganic Chemistry
SCIMAGO Q1 (2019) - 1.059 - Inorganic Chemistry

Keywords
Crystal structures Schiff base complexes Vanadium complexes Circular dichroism spectroscopy Electron spin resonance spectroscopy