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Publication details

Document type
Book chapters


Title
Preparation of Amyloidogenic Aggregates from EF-Hand ?-Parvalbumin and S100 Proteins

Participants in the publication
Javier Martínez (Author)
BioISI - Biosystems & Integrative Sciences Institute
Joana S. Cristóvão (Author)
BioISI - Biosystems & Integrative Sciences Institute
Rosa Sánchez (Author)
Maria Gasset (Author)
Cláudio M. Gomes (Author)
Dep. Química e Bioquímica
BIOISI - Instituto de Biossistemas e Ciências Integrativas

Scope
International

Summary
Proteins containing EF-hand helix-loop-helix-binding motifs play essential roles in calcium homeostasis and signaling pathways. These proteins have considerable structural and functional diversity by virtue of their cation-binding properties, and occur as either Ca2+-bound or Ca2+-free states with distinct aggregation propensities. That is the case among β-parvalbumins and S100 proteins, which under certain conditions undergo Ca2+-dependent self-assembly reactions with the formation of oligomers, amyloid-type aggregates and fibrils. These phenomena may be particularly relevant in human S100A6 protein and in fish Gad m 1 allergenic protein, which are implicated in human disease processes. Here, we describe detailed methods to generate and monitor the formation of amyloidogenic assemblies and aggregates of these two EF-hand proteins in vitro.

Editor(s)
Sigurdsson E., Calero M., Gasset M. (eds)

Date of Publication
2018

Institution
BioISI - Biosystems & Integrative Sciences Institute

Where published
Methods in Molecular Biology - Amyloid Proteins

Publication Identifiers
ISSN - 1064-3745
ISBN - 9781493978151,9781493978168

Publisher
Humana Press

Collection
Methods in Molecular Biology

Edition
2nd
Volume
1779

Number of pages
12
Starting page
167
Last page
179

Document Identifiers
DOI - https://doi.org/10.1007/978-1-4939-7816-8_11
URL - http://dx.doi.org/10.1007/978-1-4939-7816-8_11

Rankings
SCIMAGO Q3 (2018) - 0.605 - Molecular Biology

Keywords
Amyloids Calcium EF-hand Fish allergens Gad m 1 S100A6


Export

APA
Javier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset, Cláudio M. Gomes, (2018). Preparation of Amyloidogenic Aggregates from EF-Hand ?-Parvalbumin and S100 Proteins. Methods in Molecular Biology - Amyloid Proteins, 167-179

IEEE
Javier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset, Cláudio M. Gomes, "Preparation of Amyloidogenic Aggregates from EF-Hand ?-Parvalbumin and S100 Proteins" in Methods in Molecular Biology - Amyloid Proteins, 2018, pp. 167-179

BIBTEX
@incollection{41710, author = {Javier Martínez and Joana S. Cristóvão and Rosa Sánchez and Maria Gasset and Cláudio M. Gomes}, title = {Preparation of Amyloidogenic Aggregates from EF-Hand ?-Parvalbumin and S100 Proteins}, booktitle = {Methods in Molecular Biology - Amyloid Proteins}, year = 2018, pages = {167-179}, address = {}, publisher = {Humana Press} }