Tipo
Capítulo em Livro
Título
Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts.
Participantes na publicação
Sales C.R.G. (Author)
Degen G.E. (Author)
da Silva A.B. (Author)
Dep. Biologia Vegetal
BioISI
Carmo-Silva E. (Author)
Resumo
RuBisCO plays a central role in photosynthesis and, due to its catalytic inefficiencies, frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies. The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter, a spectrophotometric assay is described for measuring RuBisCO activity and activation state in leaf extracts. Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories. Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved RuBisCO function.
Editor
Covshoff S.
Instituição
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
Suporte
Photosynthesis Research Protocols
Identificadores da Publicação
Local
New York
Editora
Humana Press
Coleção
Methods in Molecular Biology
Número de Páginas
9
Página Inicial
339
Página Final
347
Identificadores do Documento
DOI -
https://doi.org/doi.org/10.1007/978-1-4939-7786-4_14
ISBN - Print ISBN 978-1-4939-7785-7
ISBN - Online ISBN 978-1-4939-7786-4
Identificadores de Qualidade
SCIMAGO Q3 (2018) - 0.605 - Biochemistry, Genetics and Molecular Biology (miscellaneous)
SCIMAGO Q3 (2020) - 0.711 - Molecular Biology