BIBLIOS

Document type
Journal articles

Document subtype
Full paper

Title
Free Superoxide is an Intermediate in the Production of H2O2 by Copper(I)-Abeta Peptide and O2

Participants in the publication
Karine Reybier (Author)
Sara Ayala (Author)
Bruno Alies (Author)
João V. Rodrigues (Author)
Susana Bustos?Rodriguez (Author)
Giovanni La?Penna (Author)
Fabrice Collin (Author)
Cláudio M. Gomes (Author)
Dep. Química e Bioquímica
BioISI
Christelle Hureau (Author)
Peter Faller (Author)

Summary
Oxidative stress is considered as an important factor and an early event in the etiology of Alzheimer's disease (AD). Cu bound to the peptide amyloid?β (Aβ) is found in AD brains, and Cu?Aβ could contribute to this oxidative stress, as it is able to produce in vitro H2O2 and HO. in the presence of oxygen and biological reducing agents such as ascorbate. The mechanism of Cu?Aβ?catalyzed H2O2 production is however not known, although it was proposed that H2O2 is directly formed from O2 via a 2?electron process. Here, we implement an electrochemical setup and use the specificity of superoxide dismutase?1 (SOD1) to show, for the first time, that H2O2 production by Cu?Aβ in the presence of ascorbate occurs mainly via a free O2.− intermediate. This finding radically changes the view on the catalytic mechanism of H2O2 production by Cu?Aβ, and opens the possibility that Cu?Aβ?catalyzed O2.− contributes to oxidative stress in AD, and hence may be of interest.


Institution
BioISI - Biosystems & Integrative Sciences Institute

Where published
Angewandte Chemie International Edition

Publication Identifiers
ISSN - 1433-7851

Publisher
Wiley



Document Identifiers
DOI - https://doi.org/10.1002/anie.201508597
URL - http://dx.doi.org/10.1002/anie.201508597

Rankings
Web Of Science Q1 (2017) - 12.102 - CHEMISTRY, MULTIDISCIPLINARY - SCIE
SCIMAGO Q1 (2016) - 5.954 - Chemistry (miscellaneous)
SCOPUS Q1 (2016) - 5.954 - General Chemistry

Keywords
Alzheimer's disease amyloid peptide bioinorganic chemistry copper reactive oxygen species