BIBLIOS

Document type
Journal articles

Document subtype
Full paper

Title
Phytochemical Characterization and Biological Evaluation of the Aqueous and Supercritical Fluid Extracts from Salvia sclareoides Brot

Participants in the publication
Daniela Batista (Author)
Dep. Química e Bioquímica
Pedro L. Falé (Author)
Maria L. Serralheiro (Author)
Dep. Química e Bioquímica
CQB
BioISI
Maria-Eduarda Araújo (Author)
Dep. Química e Bioquímica
CQB
Catarina Dias (Author)
Isabel Branco (Author)
Clara Grosso (Author)
José Coelho (Author)
António Palavra (Author)
Paulo J. A. Madeira (Author)
Alice Martins (Author)
Amélia P. Rauter (Author)
Dep. Química e Bioquímica
CQB

Summary
Plants belonging to the genus Salvia (Lamiaceae) are known to have a wide range of biological properties. In this work, extracts obtained from the aerial parts of Salvia sclareoides Brot. were evaluated to investigate their chemical composition, toxicity, bioactivity, and stability under in vitro gastrointestinal conditions. The composition of the supercritical fluid extract was determined by GC and GC-MS, while the identification of the infusion constituents was performed by HPLC-DAD and LC-MS. The in vitro cytotoxicity of both extracts (0-2 mg/mL) was evaluated in Caco-2 cell lines by the MTT assay. The anti-inflammatory and anticholinesterase activities were determined through the inhibition of cyclooxygenase-1 and acetylcholinesterase enzymes, while β-carotene/linoleic acid bleaching test and the DPPH assays were used to evaluate the antioxidant activity. The infusion inhibited cyclooxygenase-1 (IC50 = 271.0 µg/mL), and acetylcholinesterase (IC50 = 487.7 µg/ mL) enzymes, also demonstrated significant antioxidant properties, as evaluated by the DPPH (IC50 = 10.4 µg/mL) and β-carotene/linoleic acid (IC50 = 30.0 µg/mL) assays. No remarkable alterations in the composition or in the bioactivities of the infusion were observed after in vitro \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ndigestion, which supports the potential of S. sclareoides as a source of bioactive ingredients with neuroprotective, anti-inflammatory and antioxidant properties.


Institution
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA

Where published
Open Chemistry

Publication Identifiers
ISSN - 2391-5420

Publisher
Walter de Gruyter GmbH


Document Identifiers
DOI - https://doi.org/10.1515/chem-2017-0011
URL - http://dx.doi.org/10.1515/chem-2017-0011

Rankings
SCIMAGO Q2 (2017) - 0,349 - Chemistry (miscellaneous)

Keywords
Salvia sclareoides GC-MS HPLC bioactivity in vitro digestion

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