BIBLIOS

Tipo
Artigos em Revista

Tipo de Documento
Artigo Completo

Título
Botryococcus braunii and Nannochloropsis oculata extracts inhibit cholinesterases and protect human dopaminergic SH-SY5Y cells from H2O2-induced cytotoxicity

Participantes na publicação
L. Custódio (Author)
F. Soares, (Author)
H. Pereira (Author)
M. J. Rodrigues (Author)
L. Barreira (Author)
A. P. Rauter (Author)
Dep. Química e Bioquímica
CQB
F. Alberício (Author)
J. Varela (Author)

Resumo
Extracts of Botryococcus braunii and Nannochloropsis oculata were evaluated for inhibitory activity against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (TYRO) and capacity to attenuate\\\\\\\\nhydrogen peroxide (H2O2)-induced injury in the human dopaminergic cell line SH-SY5Y. We also report the antioxidant\\\\\\\\nactivity, the total phenolic content (TPC) and the fatty acid (FA) profile of these microalgae. Both species had low levels\\\\\\\\nof TPC and considerable amounts of polyunsaturated fatty acids (PUFA). The highest radical scavenging activity (RSA)\\\\\\\\nagainst 1,1-diphenyl-2-picrylhydrazyl (DPPH) was observed in the acetone extract of B. braunii and in the diethyl ether\\\\\\\\nextracts of both strains. The acetone extract of B. braunii had the highest RSA against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid). The extracts had a higher capacity to chelate iron than copper, and the highest iron chelation was achieved with the hexane extract of N. oculata. The diethyl ether and water extracts of the latter species also displayed the highest copper chelation. Except for the acetone extract of B. braunii and the water extract of N. oculata, all samples inhibited AChE, especially the hexane extract of N. oculata. Samples had moderate BChE inhibition and no effect towards TYRO. Almost all samples effectively protected neuronal cells against oxidative stress induced by H2O2. These results suggest possible novel applications of biomass from those microalgae in the pharmaceutical industry and/or as functional foods.


Instituição
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA

Suporte
JOURNAL OF APPLIED PHYCOLOGY

Identificadores da Publicação
ISSN - 0921-8971

Editora
Springer Science and Business Media LLC



Identificadores do Documento
URL - http://dx.doi.org/10.1007/s10811-014-0369-4
DOI - https://doi.org/10.1007/s10811-014-0369-4

Identificadores de Qualidade
Web Of Science Q1 (2018) - 2.635 - Marine and Fresh Water Biology

Keywords
CHE inhibitors Functional foods Microalgae Neuroprotection Oxidative stress

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