Tipo
Artigos em Revista
Tipo de Documento
Artigo Completo
Título
Purification and Characterization of Vanadium Haloperoxidases from the Brown Alga Pelvetia canaliculata.
Participantes na publicação
G. Almeida (Author)
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
M. Humanes (Author)
Dep. Química e Bioquímica
CQB
J. A. Silva (Author)
INSTITUTO SUPERIOR TÉCNICO
R. Melo (Author)
Dep. Biologia Vegetal
CO
MARE
J. J. R. Fraústo da Silva (Author)
INSTITUTO SUPERIOR TÉCNICO
R. Wever. (Author)
Resumo
Two enzymes characterised as iodoperoxidases (PcI and PcII), with vanadium-dependent activity, have been purified from the brown alga Pelvetia canaliculata (L.) Decne et Thur. (Fucaceae, Phaeophyceae), collected in the Northern Portuguese coast, at Viana do Castelo. The relative molecular masses were 166 kDa for PcI and 416 kDa for PcII, as determined by gel filtration. SDS-PAGE shows that PcI has just one band corresponding to a subunit of 66 kDa, while PcII shows four bands (66, 72, 157 and 280 kDa). The following kinetic parameters have been determined from a steady-state analysis of the oxidation of iodide by H2O2: PcI, pHopt = 6.0, mM, [mu]M, mM; and PcII, pHopt = 6.5, mM, KM(H2O2) = 20 [mu]M and mM. These iodoperoxidases are thermostable, as also observed for vanadium bromo- and chloroperoxidases.
Suporte
PHYTOCHEMISTRY
Identificadores da Publicação
ISSN - 0031-9422
Página Inicial
5
Página Final
11
Identificadores do Documento
DOI -
https://doi.org/10.1016/s0031-9422(99)00602-0
Identificadores de Qualidade
SCIMAGO Q1 (2019) - 0.763 - Plant Science
Web Of Science Q1 (2019) - 3.044 - PLANT SCIENCES - SCIE
SCOPUS Q1 (2019) - 4.9 - Plant Science