Document type
														Journal articles											
			
												
Document subtype
					Full paper
						 										
			
			
			
Title
			Evaluation of two Portuguese strains of Botryococcus braunii as biofuel feedstock
			
			
				Participants in the publication
			
																			Tavares Joao (Author)
					
																		
								Dep. Biologia Vegetal
							
						
					
																				Pardal Tiago (Author)
												
					
					
																				
Melo Ricardo (Author)
					
																		
								Dep. Biologia Vegetal
							
													
								MARE
							
						
					
							
			
							Summary
				ACOI 58 and ACOI 1257, two different Portuguese strains of colonial green microalga Botryococcus bruanii Kutz obtained from Coimbra Collection of Algae (ACOI), were evaluated on their potential for biofuel production, which was assessed from batch culture in photobioreactors under indoors and outdoors conditions using pretreated waste water and semisynthetic culture media. In the experiments, the maximum specific growth rate achieved was 0.4 day-1 with doubling times ranging from 2 to 7 days in the fastest growing phase. However, both strains showed ability to absorb nutrients in waste water cultures, to grow and accumulate oil. The maximum lipid extraction on a dry matter basis was 36% in CHU media and 29% in waste water cultures, which revealed the promising potential of these B. braunii isolates to be used in biofuel production applications. This is also supported by the predominance of oleic (C18:1, 42 to 63%) and palmitic acid (C16:0, 8 to 18%) in the lipid extract, since the methyl esters of these fatty acids (FAME) are ideal for biodiesel production. In summary, although these strains of B. braunii have presented a typical growth for this species, they produced considerable lipid content and were able to grow in waste water and under outdoors conditions that warrants further investment in their study.				
				
			
			
			
																	
						
															Date of Publication							
						
						2017-09-30					
							 
			
																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																	
							Institution
																FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																																									
				
				
			
							
					Where published
				
				International Journal of Biotechnology and Molecular Biology Research				
			
			
Publication Identifiers
			ISSN - 2141-2154			
			
			
							
Publisher
				Academic Journals				
				
			
			
							
				
			
							
											
							Number of pages
							9						
					
											
							Starting page
							20						
					
											
							Last page
							29						
					
				 
				
			
							Document Identifiers
				URL - 
https://academicjournals.org/journal/IJBMBR/article-abstract/99E48FE66109
					DOI - 
https://doi.org/10.5897/ijbmbr2017.0274
						URL - 
http://dx.doi.org/10.5897/ijbmbr2017.0274
					
				
			
			
			
							Keywords
									Wastewater
									Biofuels
									Photobioreactors
									Botryococcus braunii
									Microalgae