Document type
Book chapters
Title
Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts.
Participants in the publication
Sales C.R.G. (Author)
Degen G.E. (Author)
da Silva A.B. (Author)
Dep. Biologia Vegetal
BioISI
Carmo-Silva E. (Author)
Summary
RuBisCO plays a central role in photosynthesis and, due to its catalytic inefficiencies, frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies. The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter, a spectrophotometric assay is described for measuring RuBisCO activity and activation state in leaf extracts. Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories. Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved RuBisCO function.
Editor(s)
Covshoff S.
Institution
FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DE LISBOA
Where published
Photosynthesis Research Protocols
Publication Identifiers
Address
New York
Publisher
Humana Press
Collection
Methods in Molecular Biology
Number of pages
9
Starting page
339
Last page
347
Document Identifiers
DOI -
https://doi.org/doi.org/10.1007/978-1-4939-7786-4_14
ISBN - Print ISBN 978-1-4939-7785-7
ISBN - Online ISBN 978-1-4939-7786-4
Rankings
SCIMAGO Q3 (2018) - 0.605 - Biochemistry, Genetics and Molecular Biology (miscellaneous)
SCIMAGO Q3 (2020) - 0.711 - Molecular Biology