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Publication details

Document type
Books

Document subtype
Research results book

Title
Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts. In: Covshoff S. (eds) Photosynthesis. Methods in Molecular Biology

Participants in the publication
Sales C.R.G. (Author)
Degen G.E. (Author)
da Silva A.B. (Author)
Dep. Biologia Vegetal
BIOISI - Instituto de Biossistemas e Ciências Integrativas
Carmo-Silva E. (Author)

Summary
RuBisCO plays a central role in photosynthesis and, due to its catalytic inefficiencies, frequently limits CO2 assimilation in fully illuminated leaves at the top of unstressed crop canopies. The CO2-fixing enzyme is heavily regulated and not all the enzyme present in the leaf is active at any given moment. In this chapter, a spectrophotometric assay is described for measuring RuBisCO activity and activation state in leaf extracts. Most of the assay components are available commercially and others can be produced by established protocols, making adoption of the assay achievable by most plant biochemistry laboratories. Its relative high-throughput capacity enables large-scale experiments aimed at screening germplasm for improved RuBisCO function.

Editor(s)
Robert Carpentier

Date of Publication
2018

Where published
Methods in Molecular Biology

Publication Identifiers
ISSN - 1064-3745
eISSN - 1940-6029
ISBN - 9781607619246,9781607619253

Address
New York

Publisher
Humana Press

Collection
Methods in Molecular Biology

Edition
2nd
Volume
1770

Number of pages
9
Starting page
339
Last page
347

Document Identifiers
URL - http://dx.doi.org/10.1007/978-1-60761-925-3
DOI - https://doi.org/10.1007/978-1-60761-925-3

Rankings
SCIMAGO Q3 (2018) - 0.605 - Biochemistry, Genetics and Molecular Biology (miscellaneous)


Export

APA
Sales C.R.G., Degen G.E., da Silva A.B., Carmo-Silva E., (2018). Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts. In: Covshoff S. (eds) Photosynthesis. Methods in Molecular Biology

IEEE
Sales C.R.G., Degen G.E., da Silva A.B., Carmo-Silva E., "Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts. In: Covshoff S. (eds) Photosynthesis. Methods in Molecular Biology", 2018

BIBTEX
@book{41185, author = {Sales C.R.G. and Degen G.E. and da Silva A.B. and Carmo-Silva E.}, title = {Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts. In: Covshoff S. (eds) Photosynthesis. Methods in Molecular Biology}, year = 2018, publisher = {Humana Press} }